Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp
© Haraguchi et al. 2015
Received: 22 June 2015
Accepted: 4 December 2015
Published: 16 December 2015
An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology.
In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system.
In the automated patch-clamp measurements performed here, Kv currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations (IC50) of those blockers were in good agreement with previous reports.
The high-throughput patch-clamp system has a high potential in the field of pharmacology, toxicology, and cardiac physiology, and will contribute to the acceleration of pharmaceutical drug development and drug safety testing.
KeywordsElectrophysiology hERG channel High-throughput screening Automated patch-clamp
At present although the cost of pharmaceutical drug development has been progressing, new pharmaceutical drugs finally approved and launched into the market is decreasing steadily. In 2001, while 30 % of pharmaceutical drugs, which were tested clinically, were abandoned because of the lack of efficacy, 30 % of others were also abandoned because of safety concerns such as cardiotoxicities including ion channel inhibition [1, 2]. Ion channels are major targets of pharmaceutical drugs, it is shown that more than 13 % of clinically used drugs act primarily on ion channel proteins, these drugs are estimated to be worth more than $12 billion worldwide . Therefore, the development of an in vitro electrophysiological assay system, which can detect the efficacy of candidate drugs or cardiotoxicity including arrhythmia risks is strongly demanded in the field of pharmacological development and drug safety testing. An assay system using cells expressing human ion channels has a powerful potential when it comes to reducing the amount of animal experiments. In addition, utilizing cells expressing human ion channels is expected to be an accurate assessment, because there might be different reactivity against drugs between human and animal ion channels. For example, the heartbeat of a mouse is around 600 beats per minute, which is tenfold faster than that of human beings, thus, the duration of the action potential is much shorter and ion channels have different properties . Furthermore, the usage of mammalian cells expressing human ion channel genes is more suitable than Xenopus oocytes expressing the genes, which may be less sensitive to drug inhibition [5–7].
A patch-clamp system allows for investigation of the electrophysiological function of ion channels, and was first described by Neher and Sakmann who were awarded the Nobel Prize in Medicine in 1991 [8, 9]. While the technology is an essential method in the field of pharmacology, toxicology, and cardiac physiology, a conventional patch-clamp setup is generally thought to be demanding and needs high levels of manual dexterity, knowledge and dedication of the experimenter. An automated patch-clamp system on the other hand is easy to use compared to conventional patch-clamping [10–12].
In this study a high-throughput electrophysiological screening of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) was performed utilizing an automated 384-well-patch-clamp system. hERG channel inhibition of various blockers was analyzed, and the 50 % inhibitory concentrations (IC50) were compared to literature values.
Cell culture and cell preparation for patch-clamp analysis
In this study, HEK 293-hERG cells (Merck Millipore, Billerica, MA, USA), which are HEK 293 cells stably expressing hERG, and CHO-hERG cells (Merck Millipore), which are CHO cells stably expressing hERG, were used. The HEK 293 cells were cultured in an equal volume mixture of Dulbecco’s modified Eagle’s medium (DMEM) and Nutrient Mixture F-12 (Invitrogen Life Technologies, CA, USA) supplemented with 10 % fetal bovine serum (FBS) (Invitrogen Life Technologies) and 1 % penicillin/streptomycin (Invitrogen Life Technologies), and the CHO cells were cultured in Ham’s F12 medium (Invitrogen Life Technologies) supplemented with 10 % FBS and 1 % penicillin/streptomycin in a humidified 5 % CO2 atmosphere at 37 °C. For patch-clamp experiments, cultured cells on a polystyrene culture dish (Sumitomo Bakelite, Tokyo, Japan) were detached by Accutase (Innovative Cell Technologies, Inc., CA, USA) at room temperature for several minutes. A viable single cell suspension was obtained by resuspension in patch clamp solution with mild pipetting to avoid cell damage.
hERG channel blockers
In this study, five hERG channel blockers [astemizole (Sigma-Aldrich, St. Louis, MO, USA), cisapride monohydrate (Sigma-Aldrich), E-4031 hydrochloride (Wako pure chemical, Tokyo, Japan), quinidine (Sigma-Aldrich), and terfenadine (Sigma-Aldrich)] were used. Three concentrations of each drug were applied to the same cell cumulatively and sequentially. Three hundred eighty-four wells could be served with solution at the same time due to the availability of the 384 pipetting head of the system.
Measurement of Kv currents in an automated 384-well-patch-clamp system
A patch-clamp investigation of HEK 293 cells or CHO cells expressing hERG was performed in an automated way by using a 384-well-patch-clamp system. For the analysis, 10 μL of suspended cells (cell density: approximately 1 × 106 cells/mL) were applied to each well. A complete experiment took approximately 15–25 min. Kv currents were efficiently detected in the voltage-clamp mode of the high-throughput system (Fig. 1b, c).
High-throughput assay on hERG inhibitors
IC50 of hERG channel blockers
Data in this study
Previous data (References)
6.85 nM (n = 23)
0.9 nM (2), 26 nM (4)
21.2 nM (n = 24)
44 nM (1), 6.9 nM (4), 23-27 nM (5)
22.6 nM (n = 30)
18.1 nM (4), 12-17 nM (5)
376 nM (n = 26)
410 nM (3), 820-1,070 nM (5), 750 nM (6)
41.7 nM (n = 22)
56 nM (1), 6.6-8.4 nM (5), < 52 nM (7)
This study showed data of an automated patch-clamp system, which records ion channel currents of up to 384 cells simultaneously. The system could detect hERG channel inhibition in a high-throughput format using HEK 293 cells overexpressing hERG channels. The hERG channel is characterized as a voltage-gated inwardly rectifying potassium channel [13, 14], and plays a key role in cardiac pathology because the gene links to long QT syndrome, which is a hereditary disease causes lethal ventricular arrhythmias [15–17]. Importantly, the channel inhibition causes a drug-induced QT prolongation and is increasing the risk of sudden cardiac death [5, 15–21]. Of drugs recently removed from the market in the United States, one of the most common causes has been QT prolongation-related cardiotoxicity . Therefore, an optimal evaluation system of hERG channel blockers is important for detecting the cardiotoxicity of candidate drugs. hERG channel screening of candidate drugs at an early stage in the drug development process is accelerating the whole drug discovery procedure. This study is proposing a high-throughput screening system for investigating hERG channel inhibition using an automated multi-well-patch-clamp technology. The patch-clamp method allows for the simultaneous assessment of ion channel inhibition activity of e.g. up to 48 or 128 kinds of candidate drugs, in the case of n = 8 or 3, respectively.
It is commonly thought that the usage of human cardiomyocytes is also important in the field of pharmacological development and drug safety testing [2, 4, 23]. Human induced pluripotent stem cells (hiPSC) can efficiently differentiate into cardiomyocytes in vitro . We developed a suspension culture system, which can produce large numbers of hiPSC-derived cardiomyocytes . hiPSC-derived cardiomyocytes have been applied for cardiac regenerative medicine and the transplantation of an enormous number of the cells will contribute to positive clinical therapeutic effects . At the same time those cardiomyocytes will be also an optimal cell source for the high-throughput investigation of ion channel inhibition and thus the detection of cardiotoxicity of drugs. Our previous report showed that hiPSC-derived cardiomyocytes expressed various cardiac cell-related genes, including hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4), myosin light chain-2a (MLC-2a), MLC-2v, and Iroquoishomeobox 4 (IRX4) . HCN4 is expressed in cardiac pacemaker cells . MLC-2a is a marker of atrial myocytes, and MLC-2v and IRX4 are those of ventricular myocytes . Thus, the data suggest that hiPSC-derived differentiated cells contained various types of cardiomyocytes including pacemaker cells, atrial and ventricular myocytes. Currently, we are performing the patch-clamp analysis of hiPSC-derived differentiated cardiomyocytes, the amount of cells being expanded abundantly by the suspension culture system, using the automated 384-well-patch-clamp system. An upgrade of the here utilized 384-well-patch-clamp system to not only having the capability of performing voltage-clamp, but also current-clamp recordings is momentarily under development. With this system the effect of candidate drugs on the duration of the action potentials will be investigated, which could be translated into e.g. a prolongation of QT intervals. Additionally, the system will allow high-throughput recordings of cardiac subtypes including pacemaker cells, atrial myocytes, ventricular myocytes, and also will allow for investigating the maturation status of hiPSC-derived cardiomyocytes. Those data will contribute to the field of cardiac electrophysiology and cardiac regenerative medicine as well as pharmaceutical development.
This study shows data from a hERG screening assay in an automated high-throughput patch-clamp system. We are confident that the method will have great impact in the field of pharmacology, toxicology, and cardiac electrophysiology, also in the light of the CIPA (Comprehensive In Vitro Pro-Arrhythmia Assay) proposal that aims to define a new, integrated preclinical in vitro/in silico paradigm in which the potential proarrhythmic risk of a new drug would be assessed using not only hERG patch clamp investigations, but multiple ion channel investigations (e.g. Nav1.5 and Cav1.2). Thus, the system will contribute to the acceleration of pharmaceutical drug-development and drug-safety testing.
We thank Merck Millipore (Billerica, MA, USA) for kindly supplying HEK 293-hERG and CHO-hERG cells. We also thank Dr. Sonja Stölzle-Feix (Nanion Technologies GmbH) for her useful comments and editing assistance. This work was supported by a grant from Formation of Innovation Center for Fusion of Advanced Technologies in the Special Coordination Funds for Promoting Science and Technology “Cell Sheet Tissue Engineering Center (CSTEC)” from the Ministry of Education, Culture, Sports Science, and Technology (MEXT), Japan, and Nanion Technologies GmbH.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Kola I, Landis J. Can the pharmaceutical industry reduce attrition rates? Nat Rev Drug Discov. 2004;3:711–5.PubMedGoogle Scholar
- Laustriat D, Gide J, Peschanski M. Human pluripotent stem cells in drug discovery and predictive toxicology. Biochem Soc Trans. 2010;38:1051–7.PubMedGoogle Scholar
- Clare JJ. Targeting ion channels for drug discovery. Discov Med. 2010;9:253–60.PubMedGoogle Scholar
- Priori SG, Napolitano C, Di Pasquale E, Condorelli G. Induced pluripotent stem cell-derived cardiomyocytes in studies of inherited arrhythmias. J Clin Invest. 2013;123:84–91.PubMedPubMed CentralGoogle Scholar
- Lacerda AE, Kramer J, Shen KZ, Thomas D, Brown AM. Comparison of block among cloned cardiac potassium channels by non-antiarrhythmic drugs. Eur Heart J Supplements. 2001;3:K23–30.Google Scholar
- Nakaya H. Electropharmacological assessment of the risk of drug-induced long-QT syndrome using native cardiac cells and cultured cells expressing HERG channels. Folia Pharmacologica Japonica. 2003;121:384–92.PubMedGoogle Scholar
- Vonderlin N, Fischer F, Zitron E, Seyler C, Scherer D, Thomas D, et al. Anesthetic drug midazolam inhibits cardiac human ether-à-go-go-related gene channels: mode of action. Drug Des Devel Ther. 2015;9:867–77.PubMedPubMed CentralGoogle Scholar
- Neher E, Sakmann B. Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature. 1976;260:799–802.PubMedGoogle Scholar
- Verkhratsky A, Parpura V. History of electrophysiology and the patch clamp. Methods Mol Biol. 2014;1183:1–19.PubMedGoogle Scholar
- Xu SZ, Sukumar P, Zeng F, Li J, Jairaman A, English A, et al. TRPC channel activation by extracellular thioredoxin. Nature. 2008;451:69–72.PubMedPubMed CentralGoogle Scholar
- Milligan CJ, Li J, Sukumar P, Majeed Y, Dallas ML, English A, et al. Robotic multiwell planar patch-clamp for native and primary mammalian cells. Nat Protoc. 2009;4:244–55.PubMedPubMed CentralGoogle Scholar
- Stoelzle S, Obergrussberger A, Brüggemann A, Haarmann C, George M, Kettenhofen R, et al. State-of-the-art automated patch clamp devices: heat activation, action potentials, and high throughput in ion channel screening. Front Pharmacol. 2011;2:76.PubMedPubMed CentralGoogle Scholar
- Warmke JW, Ganeztky B. A family of potassium channel genes related to eag in Drosophila and mammals. Proc Natl Acad Sci U S A. 1994;91:3438–42.PubMedPubMed CentralGoogle Scholar
- Trudeau MC, Warmke JW, Ganetzky B, Robertson GA. HERG, a human inward rectifier in the voltage-gated potassium channel family. Science. 1995;269:92–5.PubMedGoogle Scholar
- Kirsch GE, Trepakova ES, Brimecombe JC, Sidach SS, Erickson HD, Kochan MC, et al. Variability in the measurement of hERG potassium channel inhibition: effects of temperature and stimulus pattern. J Pharmacol Toxicol Methods. 2004;50:93–101.PubMedGoogle Scholar
- Vandenberg JI, Perry MD, Perrin MJ, Mann SA, Ke Y, Hill AP. hERG K(+) channels: structure, function, and clinical significance. Physiol Rev. 2012;92:1393–478.PubMedGoogle Scholar
- Jiménez-Vargas JM, Restano-Cassulini R, Possani LD. Toxin modulators and blockers of hERG K(+) channels. Toxicon. 2012;60:492–501.PubMedGoogle Scholar
- Weirich J, Antoni H. Rate-dependence of antiarrhythmic and proarrhythmic properties of class I and class III antiarrhythmic drugs. Basic Res Cardiol. 1998;93 Suppl 1:125–32.PubMedGoogle Scholar
- Yap YG, Camm AJ. Arrhythmogenic mechanisms of non-sedating antihistamines. Clin Exp Allergy. 1999;29 Suppl 3:174–81.PubMedGoogle Scholar
- Brown AM, Rampe D. Drug-induced long QT syndrome: Is HERG the root of all evil? Pharmaceutical News. 2000;7:15–20.Google Scholar
- Tseng GN. I(Kr): the hERG channel. J Mol Cell Cardiol. 2001;33:835–49.PubMedGoogle Scholar
- Katchman AN, Koerner J, Tosaka T, Woosley RL, Ebert SN. Comparative evaluation of HERG currents and QT intervals following challenge with suspected torsadogenic and nontorsadogenic drugs. J Pharmacol Exp Ther. 2006;316:1098–106.PubMedGoogle Scholar
- Otsuji TG, Minami I, Kurose Y, Yamauchi K, Tada M, Nakatsuji N. Progressive maturation in contracting cardiomyocytes derived from human embryonic stem cells: Qualitative effects on electrophysiological responses to drugs. Stem Cell Res. 2010;4:201–13.PubMedGoogle Scholar
- Haraguchi Y, Shimizu T, Yamato M, Okano T. Cell therapy and tissue engineering for cardiovascular disease. Stem Cells Transl Med. 2012;1:136–41.PubMedPubMed CentralGoogle Scholar
- Matsuura K, Wada M, Shimizu T, Haraguchi Y, Sato F, Sugiyama K, et al. Creation of human cardiac cell sheets using pluripotent stem cells. Biochem Biophys Res Commun. 2012;425:321–7.PubMedGoogle Scholar
- Haraguchi Y, Matsuura K, Shimizu T, Yamato M, Okano T. Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering. J Tissue Eng Regen Med. 2015;9:1363–75.PubMedGoogle Scholar
- Ishii TM, Takano M, Xie LH, Noma A, Ohmori H. Molecular characterization of the hyperpolarization-activated cation channel in rabbit heart sinoatrial node. J Biol Chem. 1999;274:12835–9.PubMedGoogle Scholar
- Lin B, Kim J, Li Y, Pan H, Carvajal-Vergara X, Salama G, et al. High-purity enrichment of functional cardiovascular cells from human iPS cells. Cardiovasc Res. 2012;95:327–35.PubMedPubMed CentralGoogle Scholar