IL-1β and IL-6 are differentially released following single and multiple exposures to N-9. Female Swiss Webster mice were exposed once or once daily over 4 days to unformulated 1% N-9 and sacrificed after the indicated post-exposure intervals following the last exposure. Prior to sacrifice, saline cervicovaginal lavages were collected from the mice and analyzed for the presence of IL-1β (A, B) or IL-6 (C, D) using Luminex technology. The absence of a bar indicates no detectable cytokine release over background levels. Error bars indicate standard deviations on replicate data points.