Atorvastatin neither alters hepatocyte respiration nor hepatocyte ATP content. Panels A-C: Cellular mitochondrial O2 consumption with and without atorvastatin. Panels A-B, O2 runs with and without 150 nM atorvastatin. Panel C, O2 runs with and without 1.0 μM atorvastatin. Panel D: Cellular ATP content and values of k
as a function of incubation time. Liver specimens from TO mice were incubated in vitro at 37°C in 50 mL KH buffer (continuously gassed with 95% O2: 5% CO2) with and without 150 nM (A-B) or 1.0 μM (C-D) atorvastatin. Cellular O2 consumption and ATP content were determined as a function of time; t = 0 corresponded to tissue collection. The lines in Panels A-C are linear fits (0.873 < R
2 < 0.955). The rate of respiration (k, μM O2 min-1) was set as the negative of the slope of [O2] vs. t. The values of k
(μM O2 min-1 mg-1) are shown at the bottom of each run. The values of k
in Panel C and the cellular ATP content of the same experiment are plotted in Panel D. Eleven independent experiments were done with the TO mice and 4 independent experiments were done with the C57Bl/6 mice. U, untreated; T, treated.