Table 1 Assay protocols and DMR signals used for similarity analysis

HEK293

0.1% DMSO in buffer, 1 hr

Ligand, 10 μM

HEK-3, 9, 30

Additional file1: Figure S1, S2

Opioid*

0.1% DMSO in buffer, 1hr

Ligand, 10 μM

Buffer-3, 9, 30

Figures1 5 6 7

HEK-MOR

10 μM ligand, 1 hr

DAMGO, 10 μM

MOR-3, 9, 30

Additional file1: Figures S2 & S3

HEK-DOR

10 μM ligand, 1 hr

DPDPE, 10 μM

DOR-3, 9, 30

Figure5

HEK-KOR

10 μM ligand, 1 hr

BRL-57532, 10 μM

KOR-3, 9, 30

Figure6

SH-SY5Y

10 μM ligand, 1 hr

DAMGO, 10 μM

5Y-3, 9, 30

Figure7

Opioid*

100 ng/ml PTX, 20 hr

Ligand, 10 μM

PTX-3, 9, 30

Figures 5 6 and7

Opioid*

400 ng/ml CTX, 20 hr

Ligand, 10 μM

CTX-3, 9, 30

Figures5 6 and7

Opioid*

10 μM forskolin, 1 hr

Ligand, 10 μM

FSK-3, 9, 30

Figures5 6 and7

Opioid*

10 μM U0126, 1 hr

Ligand, 10 μM

U0126-3, 9, 30

Figures5 6 and7

Opioid*

10 μM SB202190, 1 hr

Ligand, 10 μM

SB-3, 9, 30

Figures5 6 and7

Opioid*

10 μM SP600125, 1 hr

Ligand, 10 μM

SP-3, 9, 30

Figures5 6 and7

Opioid*

10 μM LY294002, 1 hr

Ligand, 10 μM

LY-3, 9, 30

Figures5 6 and7