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Table 1 Assay protocols and DMR signals used for similarity analysis

From: Label-free integrative pharmacology on-target of opioid ligands at the opioid receptor family

Cell Probe, pretreatment duration DMR readout Labels used in clustering Figures
HEK293 0.1% DMSO in buffer, 1 hr Ligand, 10 μM HEK-3, 9, 30 Additional file1: Figure S1, S2
Opioid* 0.1% DMSO in buffer, 1hr Ligand, 10 μM Buffer-3, 9, 30 Figures1 5 6 7
HEK-MOR 10 μM ligand, 1 hr DAMGO, 10 μM MOR-3, 9, 30 Additional file1: Figures S2 & S3
HEK-DOR 10 μM ligand, 1 hr DPDPE, 10 μM DOR-3, 9, 30 Figure5
HEK-KOR 10 μM ligand, 1 hr BRL-57532, 10 μM KOR-3, 9, 30 Figure6
SH-SY5Y 10 μM ligand, 1 hr DAMGO, 10 μM 5Y-3, 9, 30 Figure7
Opioid* 100 ng/ml PTX, 20 hr Ligand, 10 μM PTX-3, 9, 30 Figures 5 6 and7
Opioid* 400 ng/ml CTX, 20 hr Ligand, 10 μM CTX-3, 9, 30 Figures5 6 and7
Opioid* 10 μM forskolin, 1 hr Ligand, 10 μM FSK-3, 9, 30 Figures5 6 and7
Opioid* 10 μM U0126, 1 hr Ligand, 10 μM U0126-3, 9, 30 Figures5 6 and7
Opioid* 10 μM SB202190, 1 hr Ligand, 10 μM SB-3, 9, 30 Figures5 6 and7
Opioid* 10 μM SP600125, 1 hr Ligand, 10 μM SP-3, 9, 30 Figures5 6 and7
Opioid* 10 μM LY294002, 1 hr Ligand, 10 μM LY-3, 9, 30 Figures5 6 and7
  1. * Opioid receptor expressing cell lines wherein the same assay protocol was applied.