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Table 1 Effect of nicotine and a selective nicotinic antagonist on electrocardiographic parameters in isolated beating heart

From: Electrophysiological and pharmacological evaluation of the nicotinic cholinergic system in chagasic rats

Protocol Sequence PQ interval Aorta Pressure Wave
Healthy Hearts Chagasic Hearts Healthy Hearts Chagasic Hearts
AV (msec) % AV (msec) % AV (mmHg) % AV (mmHg) %
Basal 46.8±2.5 100 54.2±2.6 @ 100 14.1±2.3 100 14.4±1.4 100
Nic 1 μM A 44.9±1.7 97.2±3.0 53.0±2.5 96.2±0.3 15.8±2.6 141.0±29.72 15.6±2.2 110.7±11.2
Nic 1 μM D 47.1±2.4 101.7±4.3 53.3±3.0 95.7±1.3 13.6±1.9 124.7±28.72 13.3±1.5 96.0±10.0
Wash 1 49.9±1.9 100 53.7±2.2 100 11.6±1.6 100 12.9±1.5 100
Nic 10 μM A 48.9±2.0 98.1±2.2 53.8±2.3 100.3±1.7 16.7±1.9* 180.9±37.74 16.9±1.9* 134.8±9.8
Nic 10 μM D 45.4±2.2* 90.7±2.2 54.2±2.5 104.6±2.0 @ 14.6±1.6 159.5±31.96 13.6±1.3 112.8±8.7
Wash 2 47.9±1.9 100 55.8±2.6 100 11.8±1.2 100 12.6±1.5 100
Nic 100 μM A 48.3±2.0 101.0±2.4 57.1±2.5 109.0±1.5 @ 16.5±1.7* 150.4±17.21 14.5±1.5 120.1±9.7
Nic 100 μM D 48.7±2.0 101.4±2.9 54.5±2.6 100.5±1.2 12.8±1.6 107.1±5.75 13.4±1.5 112.7±12.6
Wash 3 48.1±2.1 100 51.2±2.6 100 12.9±1.4 100 13.1±1.2 100
MA 47.4±2.0 99.6±3.8 53.0±2.4 103.4±1.7 11.7±1.5 90.5±8.13 12.3±1.3 95.3±6.4
MA+Nic 10 μM A 48.2±2.3 100.3±2.1 52.7±2.05 104.3±1.1 12.5±2.3 92.7±9.64 11.4±1.3 88.7±6.0°
MA+Nic 10 μM D 48.6±2.9 101.2±2.8° 53.6±2.4 102.8±0.4 12.4±1.6 94.4±7.88 11.4±1.4 87.7±6.6°
Wash 4 46.6±2.4 100 53.9±2.1 100 10.3±1.5 100 11.5±1.3 100
DHβE 47.4±2.4 101.7±1.7 55.4±2.5 107.8±0.5 @ 12.4±2.1 121.9±15.10 10.7±1.2 95.4±3.7
DHβE+Nic 10 μM A 49.0±2.6 105.5±1.8° 55.8±2.5 104.7±0.6 11.2±1.8 110.0±12.80° 11.0±1.2 98.5±4.5°
DHβE+Nic 10 μM D 48.8±1.9 105.6±2.2° 55.6±2.3 106.8±0.7 10.4±1.7 99.8±8.64° 10.8±1.2 96.3±4.4
Wash 5 48.4±2.1 100 55.8±2.6 100 10.0±1.9 100 10.1±0.9 100
αBGT 50.6±2.1 104.9±2.9 55.3±2.5 97.2±0.9 @ 7.7±1.4 84.3±7.33 10.6±1.0 104.2±3.8
αBGT+Nic 10 μM A 50.3±1.9 104.4±1.9° 56.0±2.9 81.3±3.6 9.4±1.7* 96.5±5.52° 8.9±1.2 99.0±5.2° @
αBGT+Nic 10 μM D 49.4±1.8 102.6±2.4 57.4±2.6 106.6±1.7 10.3±2.1 105.2±12.35 9.7±1.2 95.4±3.8 @
Wash 6 50.3±2.1 - 57.8±2.7 - 10.4±1.0 - 9.3±1.7 -
  1. A: agonist activation period at 30 sec of perfusion; D: agonist desensitization period at 5 min of perfusion; AV: absolute values; Nic: nicotine; MA: mecamylamine; DHβE: dihydro-beta-erythroidine; αBGT: alfa-bungarotoxin; * means p < 0.05 when absolute values are compared against basal or wash periods by repeated measure ANOVA followed by Dunnet post-test; ° means p < 0.05 when % values are compared in the presence and absence of the antagonist by Wilconxon matched pairs test; @ means p < 0.05 when % values are compared between healthy and chagasic groups by Mann-Whitney test.