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Table 1 Effect of nicotine and a selective nicotinic antagonist on electrocardiographic parameters in isolated beating heart

From: Electrophysiological and pharmacological evaluation of the nicotinic cholinergic system in chagasic rats

Protocol Sequence

PQ interval

Aorta Pressure Wave

Healthy Hearts

Chagasic Hearts

Healthy Hearts

Chagasic Hearts

AV (msec)

%

AV (msec)

%

AV (mmHg)

%

AV (mmHg)

%

Basal

46.8±2.5

100

54.2±2.6 @

100

14.1±2.3

100

14.4±1.4

100

Nic 1 μM A

44.9±1.7

97.2±3.0

53.0±2.5

96.2±0.3

15.8±2.6

141.0±29.72

15.6±2.2

110.7±11.2

Nic 1 μM D

47.1±2.4

101.7±4.3

53.3±3.0

95.7±1.3

13.6±1.9

124.7±28.72

13.3±1.5

96.0±10.0

Wash 1

49.9±1.9

100

53.7±2.2

100

11.6±1.6

100

12.9±1.5

100

Nic 10 μM A

48.9±2.0

98.1±2.2

53.8±2.3

100.3±1.7

16.7±1.9*

180.9±37.74

16.9±1.9*

134.8±9.8

Nic 10 μM D

45.4±2.2*

90.7±2.2

54.2±2.5

104.6±2.0 @

14.6±1.6

159.5±31.96

13.6±1.3

112.8±8.7

Wash 2

47.9±1.9

100

55.8±2.6

100

11.8±1.2

100

12.6±1.5

100

Nic 100 μM A

48.3±2.0

101.0±2.4

57.1±2.5

109.0±1.5 @

16.5±1.7*

150.4±17.21

14.5±1.5

120.1±9.7

Nic 100 μM D

48.7±2.0

101.4±2.9

54.5±2.6

100.5±1.2

12.8±1.6

107.1±5.75

13.4±1.5

112.7±12.6

Wash 3

48.1±2.1

100

51.2±2.6

100

12.9±1.4

100

13.1±1.2

100

MA

47.4±2.0

99.6±3.8

53.0±2.4

103.4±1.7

11.7±1.5

90.5±8.13

12.3±1.3

95.3±6.4

MA+Nic 10 μM A

48.2±2.3

100.3±2.1

52.7±2.05

104.3±1.1

12.5±2.3

92.7±9.64

11.4±1.3

88.7±6.0°

MA+Nic 10 μM D

48.6±2.9

101.2±2.8°

53.6±2.4

102.8±0.4

12.4±1.6

94.4±7.88

11.4±1.4

87.7±6.6°

Wash 4

46.6±2.4

100

53.9±2.1

100

10.3±1.5

100

11.5±1.3

100

DHβE

47.4±2.4

101.7±1.7

55.4±2.5

107.8±0.5 @

12.4±2.1

121.9±15.10

10.7±1.2

95.4±3.7

DHβE+Nic 10 μM A

49.0±2.6

105.5±1.8°

55.8±2.5

104.7±0.6

11.2±1.8

110.0±12.80°

11.0±1.2

98.5±4.5°

DHβE+Nic 10 μM D

48.8±1.9

105.6±2.2°

55.6±2.3

106.8±0.7

10.4±1.7

99.8±8.64°

10.8±1.2

96.3±4.4

Wash 5

48.4±2.1

100

55.8±2.6

100

10.0±1.9

100

10.1±0.9

100

αBGT

50.6±2.1

104.9±2.9

55.3±2.5

97.2±0.9 @

7.7±1.4

84.3±7.33

10.6±1.0

104.2±3.8

αBGT+Nic 10 μM A

50.3±1.9

104.4±1.9°

56.0±2.9

81.3±3.6

9.4±1.7*

96.5±5.52°

8.9±1.2

99.0±5.2° @

αBGT+Nic 10 μM D

49.4±1.8

102.6±2.4

57.4±2.6

106.6±1.7

10.3±2.1

105.2±12.35

9.7±1.2

95.4±3.8 @

Wash 6

50.3±2.1

-

57.8±2.7

-

10.4±1.0

-

9.3±1.7

-

  1. A: agonist activation period at 30 sec of perfusion; D: agonist desensitization period at 5 min of perfusion; AV: absolute values; Nic: nicotine; MA: mecamylamine; DHβE: dihydro-beta-erythroidine; αBGT: alfa-bungarotoxin; * means p < 0.05 when absolute values are compared against basal or wash periods by repeated measure ANOVA followed by Dunnet post-test; ° means p < 0.05 when % values are compared in the presence and absence of the antagonist by Wilconxon matched pairs test; @ means p < 0.05 when % values are compared between healthy and chagasic groups by Mann-Whitney test.