Effects of CNX-011-67 treatment on insulin signaling in peripheral tissues. (A and B) At the end of treatment, adipose, muscle and liver samples from lean (1), ZDF Control (2) and ZDF rats treated with CNX-011-67 (3) were subjected to western blots for p-AKT and total AKT. Blot in figure A is the data from one animal in each group (quantification was represented as fold change across all animals in each group, n = 5). (C) ITT was performed in male ZDF rats to determine the effect of CNX-011-67 on insulin induced glucose clearance. Glucose levels were monitored for a period of 60 minutes after insulin challenge. (Open circle)-Vehicle control (Black diamonds)-CNX- 011–67 (5 mg/kg). Data in all panels are mean ± SEM (n = 7 in control group / n = 8 in treated group). Statistical comparison between control and treatment group was conducted by unpaired Student’s t test for comparison with baseline values within groups (*p < 0.05, ** p < 0.01 and *** p < 0.001).