Effect of CNX-011-67 on insulin secretion, content, ATP and GCK expression. Rat islets were cultured under normal condition (control) or with high glucose and palmitate (glucolipotoxic condition; GL) or with CNX-011-67 under GL condition for 72 h. Post 72 h, islets was treated with 2 mM glucose (LG) or 11 mM glucose (HG) for 2 h and secreted insulin was measured (A). Islets were then lysed and lysate was used for intracellular insulin content measurement (B). Data (A) and (B) are representative of ten independent experiments (N = 10, n = 4). For ATP measurements, post 72 h of glucolipotoxicity in the presence or absence of CNX-011-67, islets were incubated with either 11 mM glucose (HG) alone or with HG + CNX-011-67 for 1 h. Islets were then lysed and ATP levels were estimated (N = 1, n = 3) (C). For GCK expression studies (D), RNA was isolated post 72 h of treatment and expression of GCK mRNA was measured as described under Methods (N = 2, n = 4). Statistical analysis was performed by Students t-test (Two-way, unpaired) or One-Way ANOVA with Dunnett’s post test correction, as appropriate (*p < 0.05, ** p < 0.01 and ***p < 0.001).