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Figure 3 | BMC Pharmacology and Toxicology

Figure 3

From: Efficacy of RNA polymerase II inhibitors in targeting dormant leukaemia cells

Figure 3

KG1a responses to chemotherapeutic agents. Un-manipulated KG1a cells and cells enriched for dormancy by rapamycin pre-treatment were cultured in the presence of drugs for 48 hours whereupon net cell growth and survival was estimated using the 2,3-bis(2-methoxy-4-nitro5-sulfophenyl)-5-{(phenylamino)carbonyl}-2H-tetrazolium hydroxide (XTT) assay. (A) Dose response curves in proliferating and dormancy-enriched cells. Dark lines represent un-manipulated cells and light grey lines represent dormancy-enriched cells. (B) Percentage decrease in viable cells at 2 × IC50. Dark bars represent unmanipulated cells and unfilled bars represent dormancy-enriched treated cells. Note that for araC no IC50 for dormancy-enriched cells was reached, even at 20 times the IC50 of proliferating cells. In each experiment datapoints indicate mean and standard deviation of at least 3 independent assays.

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