Model for analysis

Datafigure #

Parameters ratios from best fits to concentrationresponse curves for orthosters at three different concentrations of allosters


b

c

d

A
_{m}


ATSM

1

4.9

3.4

3.2

1.8

EXOM

46

1.9

3.0

2.6

ATSM

2

2.8

97

11

15

EXOM *

50

17

3.0

84

ATSM

3

1.6

9.2

16

1.5

EXOM

26

35

33

3.8

 Each single parameter ratio from best fits with ATSM or EXOM is adapted from analysis of three sets of data in the literature, datafigures 1 to 3, see last section in Methods for references. Each data set consists of four concentrationresponse curves, where one curve is an orthoster concentrationresponse curve without an alloster present and the three other curves are orthoster concentrationresponses experimentally obtained at three different alloster concentrations.
 Parameters a and A
_{s} for both ATSM and EXOM were initially determined by modelfits to the basic orthoster concentrationresponse curves without an alloster present. Obtained values for a and A
_{s} were inserted in the model equations, which were then use for fitting to experimental data of the parameters b, c, d, and A
_{m} in the theories. Each number in the table is a ratio between bestfit values with the largest deviation between two of three results from fits for the single parameter to three concentrationresponse curves at different alloster concentrations.
 * For responses indicating spontaneous activity as in datafigure 2, evaluation by EXOM theory was performed by assuming a level of 9% spontaneous activity, thus fitting the EXOM distribution equation to 91% activity for all three alloster concentrations, 0.03, 0.1, and 0.3 μM ([37]). For ATSM used on datafigure 2, spontaneous activity was implemented by setting L/(1 + L) = 0.09. For datafigures 1 and 3a value of 0.01 was selected for L.
 For a more detailed explanation of how the presented parameter ratios are obtained, see last section in Methods.