Figure 1
From: Suppression of eukaryotic initiation factor 4E prevents chemotherapy-induced alopecia
Inducible and reversible suppression of eIF4E in hair follicle cells. (A) Allele configuration at Rosa26 and Col1A1 and loci of shRNA/CAGs-RIK mice designed to exhibit inducible and reversible expression of shRNAs. (B) Representative immunohistochemistry staining showing eIF4E and GFP staining in the hair follicles of vehicle-treated (-Dox) and Dox-treated (+Dox) (5 days) mice. Bar represents 50 μm. (C) Representative immunohistochemistry staining showing eIF4E and GFP staining in the hair follicles of vehicle-treated (-Dox) 4E.389/CAGs-RIK mice, doxycycline-treated (+Dox) (5 days) 4E.389/CAGs-RIK mice, and Dox-treated (5 days) 4E.389/CAGs-RIK mice that were then taken off Dox for two weeks (ON/OFF Dox). Bar represents 50 μm. (D) Representative immunohistochemistry staining showing mKate2 staining in the hair follicles of vehicle-treated (-Dox) and Dox-treated (+Dox) (5 days) 4E.389/CAGs-RIK or FLuc.1309/CAGs-RIK mice. Sections are from the experiment presented in Figure 1B. Bar represents 50 μm.