Figure 6

Suppression of eIF4F protects against chemotherapy-induced cell death in non-transformed MRC5 cells. (A) Representative Giemsa staining of MRC5 cells that had been pre-treated with nutlin-3a, Cr131-b, Hipp, 4E1RCat or 4E2RCat for 24 hours , followed by exposure to paclitaxel for 48 hrs. (B) Relative viability of MRC5 cells that had been pre-treated with nutlin-3a, Cr131-b, hippuristanol (Hipp), 4E1RCat or 4E2RCat for 24 hours and followed by exposure to 40 nM vinorelbine (VRL), 200 nM nocodazole (NOCO), or 50 nM paclitaxel (PAC) for 48 hrs. Cell counts for VRL, NOCO, and PAC were normalized to controls that had not been pre-treated. n = 3. Bars denote S.E.M. (C) Cell numbers after treatment of MRC5 cells with the indicated compounds for 24 hours and left to recover for 7 days. n = 3. Bars denote S.E.M.