Evaluation of cytotoxic or cytostatic effect. MDA-MB-231 cells treated with 25 μM Gli or vehicle for 3 or 7 days were re-seeded at low density to evaluate clonogenic capacity. After 10 days in culture, the number of colonies was determined and normalized to the number of colonies in controls. Bars show that Gli pre-treatment did not signifcantly affect clonogenic capacity. p: NS, One way ANOVA.