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Table 1 Relative distributions of bacterial phylotypes, families and genera in (i) the cecum content of mice orally exposed for 8 weeks to Cd (20 or 100 ppm) or Pb (100 or 500 ppm) salts and (ii) the fecal pellets for mice orally exposed for 8 weeks to Cd (20 ppm) or Pb (100 ppm)

From: Ecotoxicology inside the gut: impact of heavy metals on the mouse microbiome

Phylum Cd0Pb0 Cd20 Cd100 Pb100 Pb500
Cecal content      
Actinobacteria 0.25% 0.59% 1.19% 2.27% 0.25%
  (range) (0.11-0.40) (0.16-1.30) (0.36-1.84) (0.11-5.39) (0.10-0.40)
  SEM 0.049 0.210 0.272 1.113 0.161
  P value - 0.0775 0.0045 0.0537 0.478
Bacteroidetes 1.9% 2.58% 1.60% 1.47% 1.36%
  (range) s(1.29-2.28) (0.35-7.66) (0.31-4.97) (0.30-3.36) (0.8-2.40)
  SEM 0.183 1.328 0.854 0.612 0.3
  P value - 0.3131 0.3697 0.2578 0.0869
Firmicutes 97.8% 96.77% 97.2% 96.24% 98.36%
  (range) (97.4-98.4) (92.0-99.3) (93.1-98.8) (91.1-99.6) (97.9-99.0)
  SEM 0.187 1.297 1.037 1.739 0.272
  P value - 0.2269 0.2916 0.1986 0.1755
Fecal pellet      
Actinobacteria 0.30% 0.39%   0.24%  
  (range) (0.18-0.50) (0.13-0.65)   (0.08-0.42)  
  SEM 0.053 0.089   0.058  
  P value - 0.2304   0.2188  
Bacteroidetes 34.4% 38.8%   35.65%  
  (range) (12.5-50.8) (30.1-7.5)   (22.4-51.5)  
  SEM 6.56 10.84   8.65  
  P value - 0.3670   0.4553  
Firmicutes 64.7% 60.44%   63.85%  
  (range) (48.2-71.3) (47.5-90.9)   (38.2-77.5)  
  SEM 6.73 10.86   8.72  
  P value - 0.3736   0.4701  
Family Cd0Pb0 Cd20 Cd100 Pb100 Pb500
Cecal content      
Lachnospiraceae 72.6% 53.17% 25.9% 43.7% 67.5%
  (range) (33.2-88.3) (26.8-76.6) (10.3-28.5) (27.1-61.4) (59.1-75.0)
  SEM 10.01 9.24 4.15 6.09 2.62
  P value - 0.037 0.0081 0.0378 0.05859
Lactobacillaceae 22.34% 38.20% 54.68% 38.24% 26.1%
  (range) (5.6-64.9) (19.01-60.6) (41.6-81.6) (28.7-67.1) (19.2-32.1)
  SEM 10.88 8.54 7.06 7.26 2.09
  P value - 0.05859 0.0379 0.0379 0.05859
Ruminococcaceae 1.82% 2.15% 2.64% 2.15% 1.14%
  (range) (0.72-3.01) (0.76-5.91) (0.81-7.21) (0.63-3.22) (0.58-1.67)
  SEM 0.442 0.836 0.876 0.934 0.939
  P value - 0.3804 0.2627 0.3106 0.0997
Porphyromonadaceae 0.42% 0.63% 0.42% 0.49% 0.41%
  (range) (0.0-1.25) (0.0-2.24) (0.0-1.41) (0.0-1.65) (0.05-1.24)
  SEM 0.228 0.438 0.433 0.415 0.411
  P value - 0.3152 0.4774 0.4139 0.0641
Rikenellaceae 0.51% 0.42% 0.27% 0.21% 0.26%
  (range) (0.0-1.32) (0.0-1.32) (0.0-0.92) (0.0-0.6)  
  (0.0-0.47)      
  SEM 0.250 0.245 0.259 0.174 0.174
  P value - 0.3883 0.2216 0.1564 0.1800
Coriobacteriaceae 0.49% 1.19% 2.38% 4.57% 0.36%
  (range) (0.2-0.76) (0.23-2.6) (0.69-4.35) (0.2-11.75) (0.1-0.51)
  SEM 0.111 0.0939 0.107 0.433 0.138
  P value - 0.1736 0.01414 0.0250 0.3
Streptococcaceae 0.3% 0.14% 0.15% 0.24% 0.04%
  (range) (0.0-0.50) (0.05-0.3) (0.0-0.66) (0–0.75) (0–0.1)
  SEM 0.102 0.0239 0.0983 0.0894 0.0581
  P value - 0.0901 0.1905 0.3711 0.0164
Erysipelotrichaceae 0.1% 2.32% 12.98% 6.89% 3.6%
  (range) (0.0-0.24) (0.42-8.02) (3.82-28.12) (2.13-14) (1.73-6.05)
  SEM 0.044 0.232 0.219 0.268 0.02236
  P value - 0.0182 0.0096 0.0096 0.00051
Family Cd0Pb0 Cd20   Pb100  
Fecal pellet      
Lachnospiraceae 37.36% 23.67%   12.55%  
  (range) (18.8-86.9) 1.77-82.9)   (3.52-26.6)  
  SEM 12.81 14.14   17.28  
  P value - 0.0453   0.023  
Lactobacillaceae 32.99% 42.77%   50.88%  
  (range) (10.3-51.2) (4.49-67.1)   (25.1-65.6)  
  SEM 8.58 8.87   10.02  
  P value - 0.2782   0.1121  
Ruminococcaceae 1.70% 1.4%   0.83%  
  (range) (0.24-3.46) (0.08-4.58)   (0.11-1.61)  
  SEM 0.65 0.65   0.86  
  P value - 0.3916   0.1275  
Porphyromonadaceae 11.14% 13.01%   12%  
  (range) (3.46-16.25) (1.60-22.04)   (4.88-19.6)  
  SEM 2.22 3.11   3.84  
  P value - 0.3447   0.4075  
Rikenellaceae 8.36% 4.99%   5.82%  
  (range) (5.71-14.18) (1.93-16.07)   (0.45-18.5)  
  SEM 1.92 2.47   2.87  
  P value - 0.1736   0.2626  
Coriobacteriaceae 0.68% 0.67%   0.41%  
  (range) (0.03-0.99) (0.32-1.32)   (0.16-0.67)  
  SEM 0.137 0.114   0.159  
  P value - 0.4841   0.0677  
Streptococcaceae 0.27% 0.26%   0.20%  
  (range) (0.0-0.68) (0.02-0.69)   (0–0.36)  
  SEM 0.127 0.129   0.09  
  P value - 0.4969   0.3060  
Erysipelotrichaceae 0.33% 4.05%   6.56%  
  (range) (0.0-0.89) (0.48-15.53)   (0.44-20.7)  
  SEM 0.158 0.399   0.4033  
  P value - 0.0585   0.0107  
Prevotellaceae 0.14% 1.06%   0.75%  
  (range) (0.0-0.25) (0.08-4.14)   (0.11-1.22)  
  SEM 0.058 0.806   0.812  
  P value - 0.1345   0.049  
Clostridiaceae 0.00% 0.675%   1.55%  
  (range) (0.0-0.00) (0.0-2.17)   (0.0-3.06)  
  SEM 0.0 0.094   0.0940  
  P value - 0.0628   0.0086  
Genus Cd0Pb0 Cd20 Cd100 Pb100 Pb500
Cecal content      
Lactobacillus 75.28 84.57% 77.4% 74.8% 83.82%
  (range) (60–98.9) (68–91.11) (57.6-93.6) (54.1-90.6) (80.4-89.3)
  SEM 7.554 4.419 5.97 7.741 1.589
  P value - 0.2229 0.1248 0.1234 0.1503
Blautia 5.39% 2.82% 0.36% 4.90% 0.78%
  (range) (0–12.6) (0.45-8.8) (0–0.97) (0–9.2) (0.3-1.22)
  SEM 2.58 1.526 0.176 1.990 0.165
  P value - 0.2456 0.1085 0.1136 0.2036
Coprococcus 3.49% 3.06% 0.46% 1.65% 0.64%
  (range) (0–7.95) (0.22-6.49) (0.14-0.73) (0.7-3.62) (0.32-1.2)
  SEM 1.651 1.172 0.110 0.521 0.151
  P value - 0.1874 0.2086 0.2677 0.2757
Alistipes 3.42% 1.40% 0.41% 0.40% 0.74%
  (range) (0.0-12.5)(0.0-4.0) (0.0-1.49) (0.0-1.16) (0.0-1.34)  
  SEM 2.40 0.725 0.273 0.232 0.221
  P value - 0.2084 0.0439 0.4421 0.0461
Steptococcus 2.84% 0.35% 0.24% 0.47% 0.11%
  (range) (0.0-6.67) (0.11-0.80) (0.0-1.05) (0.0-1.45) (0.0-0.24)
  SEM 1.235 0.132 0.204 0.274 0.0514
  P value - 0.4189 0.0522 0.1590 0.0616
Barnesiella 1.56% 0.34% 0.44% 0.66% 0.72%
  (range) (0.0-6.67) (0.0-0.8) (0.0-1.35) (0.0-2.6) (0.0-2.27)
  SEM 1.295 0.152 0.244 0.503 0.404
  P value - 0.1598 0.4157 0.4842 0.1505
Bacteroides 0.95% 0.44% 0.09% 0.11% 0.36%
  (range) (0.0-3.33) (0.0-1.6) (0.0-0.37) (0–0.54) (0–1.29)
  SEM 0.631 0.292 0.071 0.108 0.236
  P value - 0.0401 0.0356 0.0489 0.0229
Turicibacter 0.28% 4.02% 19.63% 14.34% 11.32%
  (range) (0.0-1.15) (0.76-11.8) (4.48-41.3) (3.09-22.15) (6.37-15.4)
  SEM 0.222 1.987 6.566 4.599 1.749
  P value - 0.0492 0.0092 0.0078 0.0001
Genus Cd0Pb0 Cd20   Pb100  
Fecal pellet      
Lactobacillus 49.31% 58.0%   63.07%  
  (range) (8.96-73.18) (22.4-88.4)   (34.4-82.9)  
  SEM 10.71 12.14   11.67  
  P value - 0.3032   0.2053  
Blautia 0.73% 0.75%   0.28%  
  (range) (0.0-2.99) (0.0-3.28)   (0.0-0.64)  
  SEM 0.570 0.634   0.143  
  P value - 0.4873   0.2331  
Coprococcus 1.61% 0.83%   0.14%  
  (range) (0.0-5.97) (0.0-3.48)   (0.0-0.32)  
  SEM 1.101 0.664   0.065  
  P value - 0.2795   0.1091  
Alistipes 17.5% 8.47%   7.20%  
  (range) (7.2-29.8) (2.0-19.3)   (0.43-24.5)  
  SEM 3.871 3.822   4.454  
  P value - 0.0678   0.0495  
Steptococcus 0.50% 0.38%   0.28%  
  (range) (0.0-1.44) (0.1-0.87)   (0.0-0.5)  
  SEM 0.263 0.152   0.094  
  P value - 0.3494   0.2254  
Barnesiella 14.18% 9.13%   7.38%  
  (range) (6.8-25.3) (5.12-16.7)   (2.4-11.8)  
  SEM 3.049 2.216   1.711  
  P value - 0.1086   0.0438  
Bacteroides 11.17% 12.32%   10.46%  
(range) (4.7-19.6) (2.08-39.9)   (3.76-24.8)  
SEM 3.175 7.419   4.364  
P value - 0.4448   0.4492  
Turicibacter 0.75% 4.81%   8.71%  
  (range) (0.0-1.49) (0.82-17.6)   (2.84-28.24)  
  SEM 0.307 3.236   4.996  
  P value - 0.0483   0.0281  
  1. Data are expressed as the mean, range and SEM percentage abundance of the total assignment (n = 5 animals per group) and the corresponding p value is given in italics. The threshold for statistical significance was set to p < 0.05.