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Table 1 pEC50, Ka, Hill slopes and Emax for the activation of PKGIα by cNMPs.

From: Activation profile of cGMP-dependent protein kinase Iα

cNMP

pEC50

Ka (µM)

Hill slope

Emax

cGMP**

6.98 ± 0.04

0.11

1.71 ± 0.36

1.00 ± 0.04

2-MB-cGMP

5.84 ± 0.13

1.45

1.12 ± 0.34

0.66 ± 0.03

cAMP**

4.82 ± 0.11

15.13

1.28 ± 0.39

0.59 ± 0.05

6-MB-cAMP

4.67 ± 0.06

21.38

1.35 ± 0.19

0.81 ± 0.03

cCMP**

4.58 ± 0.14

26.30

1.84 ± 0.53

0.55 ± 0.04

4-MB-cCMP

4.05 ± 0.10

89.13

1.10 ± 0.23

0.71 ± 0.06

Sp-cAMPS

3.72 ± 0.61

190.55

1.38 ± 2.86

0.16 ± 0.01*

Rp-cAMPS

n.d.

n.d.

n.d.

n.d.

Sp-cCMPS

3.53 ± 0.97

295.12

1.11 ± 1.34

0.17 ± 0.02*

Rp-cCMPS

n.d.

n.d.

n.d.

n.d.

cIMP**

4.72 ± 0.04

19.05

1.54 ± 0.16

0.87 ± 0.02

cUMP**

4.15 ± 0.04

70.79

0.85 ± 0.21

0.72 ± 0.03

cXMP**

3.98 ± 0.04

104.71

2.06 ± 1.93

0.69 ± 0.03

  1. *: value shows the maximum activation with 3 mM cNMP without saturation of the concentration/response curve
  2. **: data from Wolter et al, Biochem Biophys Res Commun. 2011, 415: 563-566.
  3. n.d.: not determinable because of lack of saturation of the concentration/response curve.