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Table 2 The estimated LD 50 of Yessotoxin (YTX) in different developmental stages of RSP design

From: The development of response surface pathway design to reduce animal numbers in toxicity studies

Design Dose (μg/kg BW) Proportion of dead mice LD50with 95% CI (μg/kg BW)
Isotonic regression Spearman-Karber
Basic RSP 400 3/9 (0.33) 463 (383 – 535) 457 (400 – 522)
450 4/9 (0.44)
500 7/9 (0.78)
600 5/9 (0.56)
*) Included k-adjustment factor 400 3/9 (0.33) 481 (362 – 566) 480 (408 – 565)
462 4/9 (0.44)
499 5/9 (0.56)
581 5/9 (0.56)
*) Optimising use of mice 400 1/3 (0.33) 447 (378 – 504) 444 (379 – 521)
462 5/9 (0.56)
499 5/7 (0.71)
581 4/5 (0.80)
*) Multinomial decision variable with all four design levels 400 1/3 (0.33) 473 (442 – 517) 471 (430 – 516)
465 3/7 (0.43)
468 4/9 (0.44)
482 3/5 (0.60)
  1. The results are expressed with 95% Confidence Intervals. *) Simulated data based on the 36 observations in the basic RSP model.