Liver histopatholologic analysis of mice 24 h following a single IP injection of CLR01. Hepatocytes from A) vehicle-treated, and B) 10-mg/kg-treated mice show moderate amounts of glycogen vacuolation. C) Zone-1 hepatocytes from 100-mg/kg-treated mice show glycogen vacuolation. Zone-2 hepatocytes are normal sized. Zone-3 hepatocytes are pale with granular eosinophilic cytoplasm and some nuclei show pyknosis.