Brain CLR01 levels in young, middle-aged, and old WT and 3×Tg mice. CLR01 radioactivity levels are given per g of brain. Most group × time combinations fall between 10,000 - 20,000 CPM/g (marked with red lines). Double injection studies in aged WT mice show on average double the radioactivity levels of single injection group, 22 m WT. Aged WT mice dosed with 5× the amount of CLR01, show on average 5-times the radioactivity levels of the 1× group, 22 m WT. Data are given as mean ± SEM.