Table 1 Characteristics and performance data of the primers used for reference gene-stability measure M and qPCR
Gene symbol | Primer sequence (forward, reverse; from 5’ to 3’) | NCBI GeneBank | Product size (bp) | Skeletal muscle | Liver | ||||
|---|---|---|---|---|---|---|---|---|---|
Slope | R2# | Efficiency* | Slope | R2# | Efficiency* | ||||
ACADL | AAGGATTTATTAAGGGCAAGAAGC | NM_012819.1 | 380 bp | -4.45 | 1.000 | 1.68 | -3.54 | 0.999 | 1.92 |
GGAAGCGGAGGCGGAGTC | |||||||||
ACADM | CAAGAGAGCCTGGGAACTTG | NM_016986.2 | 154 bp | -3.29 | 0.998 | 2.01 | -3.29 | 0.998 | 2.01 |
CCCCAAAGAATTTGCTTCAA | |||||||||
ACADS | ACATCTCTTCCCCACATCGC | NM_022512.2 | 204 bp | -2.98 | 0.999 | 2.16 | -3.55 | 0.999 | 1.91 |
CCGAACTTCAGGATGGGTCC | |||||||||
ACOX1 | CTGGGCTGAAGGCTTTTACT | NM_017340.2 | 172 bp | -3.60 | 0.997 | 1.90 | -3.97 | 0.999 | 1.79 |
GCTGTCTGCAGCATCATAAC | |||||||||
ALDH9 | TTGAGCGGCTGCGACACGAC | NM_022273.2 | 82 bp | - | -3.42 | 0.999 | 1.96 | ||
TGACCTCGCTCCTCCGCGTA | |||||||||
ATP5B | GCACCGTCAGAACTATTGCT | NM_134364 | 203 bp | -3.58 | 0.998 | 1.90 | -3.33 | 0.999 | 2.00 |
GAATTCAGGAGCCTCAGCAT | |||||||||
BBOX1 | GGATGGGGCTCGCTTGATGCA | NM_022629.1 | 281 bp | - | -3.38 | 0.997 | 1.98 | ||
GGAGTCCTGCTCTGGCCTCCT | |||||||||
CANX | CCAGATGCAGATCTGAAGAC | NM_172008 | 175 bp | -3.30 | 1.000 | 2.01 | -3.45 | 0.999 | 1.95 |
CTGGGTCCTCAATTTCACGT | |||||||||
MDH1 | CAGACAAAGAAGAGGTTGCC | NM_033235.1 | 206 bp | -3.41 | 0.999 | 1.96 | -3.27 | 0.999 | 2.02 |
CGTCAGGCAGTTTGTATTGG | |||||||||
RPL13 | CTTAAATTGGCCACGCAGCT | XR_086310 | 198 bp | -3.20 | 0.999 | 2.05 | -3.53 | 0.997 | 1.92 |
CTTCTCAACGTCTTGCTCTG | |||||||||
SLC22A5 | GAACTCACGAGCCTCGCACGC | NM_019269.1 | 117 bp | -2.98 | 1.000 | 2.16 | -3.51 | 1.000 | 1.93 |
TCGTCGTAGTCCCGCATGCC | |||||||||
TOP1 | GAAGAACGCTATCCAGAAGG | NM_022615 | 137 bp | -3.45 | 0.999 | 1.95 | -3.34 | 0.998 | 1.99 |
GCTTTGGGACTCAGCTTCAT | |||||||||
YWHAZ | GACGGAAGGTGCTGAGAAA | NM_013011 | 198 bp | -3.11 | 0.999 | 2.10 | -3.30 | 0.999 | 2.01 |
GCAGCAACCTCAGCCAAGT | |||||||||