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Table 3 Genotoxicity evaluation criteria for the in vitro γH2AX assay by HCS

From: Characterisation of an aerosol exposure system to evaluate the genotoxicity of whole mainstream cigarette smoke using the in vitro γH2AX assay by high content screening

γH2AX response Classification
> 1.5-fold γH2AX @ RCC > 25% Genotoxic (+)
< 1.5-fold γH2AX @ RCC 100-0% Non-genotoxic (-)
> 1.5-fold γH2AX @ RCC < 25% “False” positive; Cytotoxic-driven genotoxicity (C)
1.5-fold γH2AX @ RCC ≥ 25% Equivocal (±)
  1. Table adapted from [13].