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Table 3 Genotoxicity evaluation criteria for the in vitro γH2AX assay by HCS

From: Characterisation of an aerosol exposure system to evaluate the genotoxicity of whole mainstream cigarette smoke using the in vitro γH2AX assay by high content screening

γH2AX response

Classification

> 1.5-fold γH2AX @ RCC > 25%

Genotoxic (+)

< 1.5-fold γH2AX @ RCC 100-0%

Non-genotoxic (-)

> 1.5-fold γH2AX @ RCC < 25%

“False” positive; Cytotoxic-driven genotoxicity (C)

1.5-fold γH2AX @ RCC ≥ 25%

Equivocal (±)

  1. Table adapted from [13].
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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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