BMC Pharmacology and Toxicology

Table 1 Detection limits of the different stimulants

From: Pyrogen detection methods: Comparison of bovine whole blood assay (bWBA) and monocyte activation test (MAT)

	Detection limits
	Experiment 1	Experiment 2	Experiment 3	PyroDetect System
	n = 6 – 9	n = 6	n = 5	Poolblood
LPS E.coli	0.08 EU/ml	0.04 EU/ml	0.08 EU/ml	0.04 EU/ml ‡
0127:B8	0.08 EU/ml	0.04 EU/ml	0.08 EU/ml	0.04 EU/ml ‡
LPS E.coli	not tested	0.5 EU/ml	0.25 EU/ml	0.25 EU/ml
0113:H10	not tested	0.5 EU/ml	0.25 EU/ml	0.25 EU/ml
Peptidoglycan	10 μg/ml	50 μg/ml	10 μg/ml	1 μg/ml ‡
Bac. subtilis	10 μg/ml	50 μg/ml	10 μg/ml	1 μg/ml ‡
Lipoteichoic acid	10 μg/ml	10 μg/ml	10 μg/ml	1 μg/ml ‡
Staph. aureus

Different animals were used, permutation test, compared to unstimulated blood. Experiment 3 was the comparison with the PyroDetect System. ‡ = lowest concentration tested.

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