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Table 1 Detection limits of the different stimulants

From: Pyrogen detection methods: Comparison of bovine whole blood assay (bWBA) and monocyte activation test (MAT)

  Detection limits
  Experiment 1 Experiment 2 Experiment 3 PyroDetect System
  n = 6 – 9 n = 6 n = 5 Poolblood
LPS E.coli 0.08 EU/ml 0.04 EU/ml 0.08 EU/ml 0.04 EU/ml ‡
0127:B8
LPS E.coli not tested 0.5 EU/ml 0.25 EU/ml 0.25 EU/ml
0113:H10
Peptidoglycan 10 μg/ml 50 μg/ml 10 μg/ml 1 μg/ml ‡
Bac. subtilis
Lipoteichoic acid 10 μg/ml 10 μg/ml 10 μg/ml 1 μg/ml ‡
Staph. aureus     
  1. Different animals were used, permutation test, compared to unstimulated blood. Experiment 3 was the comparison with the PyroDetect System. ‡ = lowest concentration tested.