Nobiletin's effect on NF-κB (p50) expression. (a) Effect of nobiletin on total NF-kB expression, (b) Effect of nobiletin on nuclear NF-kB expression. PC-3 and DU-145 cells (1.0 × 106) were seeded in 60 mm dishes, incubated overnight, and treated with nobiletin for 24 hours. Cells were harvested and analyzed by SDS-PAGE and Western Blotting. NF-кB protein levels whole cells were normalized by GAPDH protein levels and its protein levels of nucleus were normalized by PCNA protein levels. They were expressed as percentages of control. Data represents means ± SE from 3 independent experiments. *P < 0.05 compared to control. **P < 0.01 compared to control.