Parameter | Analysis | Results and notes |
---|---|---|
Structural and conformational characterisation | First 15 N-terminal residues by automatic Edman degradation | Results agree with the expected sequence |
Reducing and non-reducing Glu-C proteolysis and RP-HPLC/MS mapping | Primary structure and disulfide bridge positions according to the expected sequence | |
MALDI-TOF-MS | According to the calculated value | |
SDS-PAGE | Migration profile comparable to Neupogen® | |
SE-HPLC | Elution profile comparable to Neupogen® | |
Identity | Western blotting | Binding to specific antibody as Neupogen® |
RP-HPLC | Elution profile comparable to Neupogen® |