Modeling of chemical inhibition from amyloid protein aggregation kinetics

Table 3 Parametric estimates and confidence intervals (α = 0.05) from the equation ( 1) applied to the aggregation insulin increased data influenced by EGCG_1, EGCG_2, di-C7-PC and methylglyoxal concentrations

Parameters		EGCG_1	EGCG_2	di-C7-PC	Methylglyoxal
Aggregation model	X _m (AU or %)	1.12±0.03	1.28±0.06	99.85±4.91	541.20±11.38
	v _m (AU h⁻¹ or % h⁻¹)	0.18±0.01	0.25±0.04	1.55±0.27	219.68±20.98
	λ (h)	4.78±0.28	53.34±0.28	124.62±5.86	2.92±0.12
Effect on X _m	K _x	0.43±0.03	0.94±0.02	0.75±0.05	1.00±0.44
	m _x (mM)	0.68±0.08	0.01±0.00	0.65±0.12	1.80±1.25
	a _x	1.65±0.45	0.96±0.20	4.04±2.37	0.99±0.23
Effect on v _m	K _v	NS	NS	0.86±0.04	0.99±0.01
	m _v (mM)	NS	NS	0.47±0.06	0.42±0.07
	a _v	NS	NS	2.51±2.35	0.74±0.08
Effect on λ	K _λ	0.81±0.10	NS	NS	NS
	m _λ (mM)	0.50±0.08	NS	NS	NS
	a _λ	0.98±0.24	NS	NS	NS
	EC _50,τ (mM)	0.44	0.006	0.47	0.33
	τ (h)	7.65	55.02	150.68	3.92
	p-value	<0.001	<0.001	<0.001	<0.001
	B _f	0.88	0.80	1.00	1.04
	A _f	1.23	1.40	1.18	1.15
	R ² _adj	0.992	0.977	0.984	0.974

Statistical values of adjusted coefficient of multiple determination (R ² _adj) and p-values from Fisher’s F-test (α = 0.05). B _f and A _f are the bias and accuracy factor, respectively. NS: non-significant.

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