Figure 3

The AMDM mutants have markedly reduced guanylyl cyclase activity. 293 cells were transiently transfected with plasmids containing green fluorescent protein (GFP), WT-GC-B or the indicated mutants of GC-B. Crude membranes were prepared from the transfected cells and assayed for guanylyl cyclase activity under basal, CNP-stimulated or Triton X-100 and manganese-stimulated conditions. The vertical arrows in the bars and symbols represent the SEM where n = 3.