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Figure 5 | BMC Pharmacology and Toxicology

Figure 5

From: Effects of cytarabine on activation of human T cells – cytarabine has concentration-dependent effects that are modulated both by valproic acid and all-trans retinoic acid

Figure 5

Expression of the CD69 and CD38 activation markers by anti-CD3 plus anti-CD28 activated CD4+ T lymphocytes – effects of cytarabine, ATRA and valproic acid tested alone or in combination. PBMCs derived from three healthy donors were activated during in vitro culture with anti-CD3 and anti-CD28, and flow-cytometric analysis of surface CD69 and CD38 expression was performed after 20, 44, and 68 hours of culture. (A) The figure shows the mean fluorescence intensity (MFI) of surface CD69 and CD38 expression by CD4+CD5+ T lymphocytes in drug-free control cultures after 20, 44, and 68 hours (median and range, three experiments). (B) The overall results for CD69 expression are presented as bar graph (median MFI and range) for CD4+CD5+ T lymphocytes cultured in vitro for 20, 44, and 68 hours in the presence of the indicated single drugs or drug combinations. (C) The overall results for CD38 expression are presented as bar graph (median MFI and range) for CD4+CD5+ T lymphocytes cultured in vitro for 20, 44, and 68 hours in the presence of the indicated single drugs or drug combinations. Repeated measures ANOVA with Dunnett’s Multiple Comparison Test was for the statistical analyses (*p <0.05; **p <0.01; ***p <0.001).

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