Skip to main content

Table 2 Cellular amino acids’ content in 293/mock, 293/B5-11 and 293/mb5-8 cells

From: Upregulation of cellular glutathione levels in human ABCB5- and murine Abcb5-transfected cells

Amino acid

293/mock

293/B5-11

293/mb5-8

Asp

1.9 ± 0.45 (100)

2.4 ± 0.42 (130)

2.6 ± 0.40 (130)

Glu

8.3 ± 1.5 (100)

12 ± 1.3 (140)

14 ± 1.2 (170)

Gly

4.3 ± 0.28 (100)

4.9 ± 0.82 (110)

3.8 ± 0.24 (87)

Gln

54 ± 7.5 (100)

55 ± 7.5 (100)

42 ± 4.2 (77)

Thr

5.6 ± 0.86 (100)

5.7 ± 0.75 (100)

4.9 ± 0.59 (88)

Ala

0.91 ± 0.12 (100)

1.0 ± 0.19 (110)

1.4 ± 0.15 (150)

Pro

1.9 ± 0.35 (100)

1.4 ± 0.25 (72)

1.4 ± 0.20 (76)

Val

1.6 ± 0.071 (100)

1.5 ± 0.025 (94)

1.5 ± 0.075 (92)

Ile

1.6 ± 0.087 (100)

1.4 ± 0.13 (86)

1.3 ± 0.18 (83)

Leu

1.6 ± 0.23 (100)

1.3 ± 0.14 (81)

1.2 ± 0.18 (78)

Phe

1.1 ± 0.087 (100)

0.80 ± 0.058 (75)

0.78 ± 0.060 (73)

Lys

0.27 ± 0.019 (100)

0.33 ± 0.031 (120)

0.30 ± 0.063 (110)

Tyr

1.0 ± 0.19 (100)

0.57 ± 0.085 (55)

0.60 ± 0.079 (57)

  1. The methanol extracts of the cells were derivatized using 4-fluoro-7-nitro-2,1,3-benzoxadiazole and subjected to HPLC analysis. Each value represents the amino acid’s content (nmol/106 cells). Numbers in parentheses are the percentage relative to 293/mock cells. The data are presented as mean ± SD of triplicate determinations