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Table 2 Cellular amino acids’ content in 293/mock, 293/B5-11 and 293/mb5-8 cells

From: Upregulation of cellular glutathione levels in human ABCB5- and murine Abcb5-transfected cells

Amino acid 293/mock 293/B5-11 293/mb5-8
Asp 1.9 ± 0.45 (100) 2.4 ± 0.42 (130) 2.6 ± 0.40 (130)
Glu 8.3 ± 1.5 (100) 12 ± 1.3 (140) 14 ± 1.2 (170)
Gly 4.3 ± 0.28 (100) 4.9 ± 0.82 (110) 3.8 ± 0.24 (87)
Gln 54 ± 7.5 (100) 55 ± 7.5 (100) 42 ± 4.2 (77)
Thr 5.6 ± 0.86 (100) 5.7 ± 0.75 (100) 4.9 ± 0.59 (88)
Ala 0.91 ± 0.12 (100) 1.0 ± 0.19 (110) 1.4 ± 0.15 (150)
Pro 1.9 ± 0.35 (100) 1.4 ± 0.25 (72) 1.4 ± 0.20 (76)
Val 1.6 ± 0.071 (100) 1.5 ± 0.025 (94) 1.5 ± 0.075 (92)
Ile 1.6 ± 0.087 (100) 1.4 ± 0.13 (86) 1.3 ± 0.18 (83)
Leu 1.6 ± 0.23 (100) 1.3 ± 0.14 (81) 1.2 ± 0.18 (78)
Phe 1.1 ± 0.087 (100) 0.80 ± 0.058 (75) 0.78 ± 0.060 (73)
Lys 0.27 ± 0.019 (100) 0.33 ± 0.031 (120) 0.30 ± 0.063 (110)
Tyr 1.0 ± 0.19 (100) 0.57 ± 0.085 (55) 0.60 ± 0.079 (57)
  1. The methanol extracts of the cells were derivatized using 4-fluoro-7-nitro-2,1,3-benzoxadiazole and subjected to HPLC analysis. Each value represents the amino acid’s content (nmol/106 cells). Numbers in parentheses are the percentage relative to 293/mock cells. The data are presented as mean ± SD of triplicate determinations