Fig. 2
Effects of Cd on heme-oxygenase-1 expression. HepG2 cells were (A) treated with 5 and 10 μM CdCl2 for 24 h and (B) pre-treated with 10 μM CoPPIX for 24 h before exposure to 5 and 10 μM CdCl2 for 24 h. The expression of HO-1 protein was determined in the whole cell lysates by western blot analysis and GAPDH was used as loading control. Data represent the mean ± SD of three different experiments (n = 3). Significant difference between Cd-treated and control (***p < 0.0001). #p < 0.01 as significant difference between CoPPIX pre-treated and non pre-treated cells