Fig. 3

Composite images were created through GNU Image Manipulation Program (GIMP 2) which was used to identify myoblasts (defined as cells with one or two nuclei) or myotubes (defined as cells with three or more nuclei). Phase contrast images were captured to visualize myotubes and myoblasts (a), while fluorescent images were captured to label nuclei with dots (b) and visualize nuclei with DAPI (c). These layers were combined into a composite image (d). An example of a composite image with nuclei in myoblasts labeled with yellow (large) dots and nuclei in myotubes labeled with red (small) dots to allow for quantification of fraction of nuclei in myotubes is included (e). Scale bar = 100 μm