Fig. 4
From: Sodium nitrate decreases agrin-induced acetylcholine receptor clustering
NaNO3 decreased myogenin and AChR gene expression in correlation with the agrin-induced AChR clustering data. Untreated agrin-induced cultures were compared with cultures exposed to 10 ng/mL, 1 μg/mL, or 100 μg/mL NaNO3 for the last 48 h of 72 h in DM. Cultures were assayed for the amount of myogenin or AChR protein and compared with an actin control. The data correlating these two effects of NaNO3 is detailed in Table 2. These results demonstrate that NaNO3 decreases the frequency of agrin-induced AChR clustering by a mechanism that causes decreased myogenin and AChR gene expression