No. of subjects
|
16
|
69
|
38
|
16
| |
Gender (% female)
|
81
|
35
|
29
|
43
|
0.003
|
Age (years)
|
42 (25.5–53.5)
|
53 (41.2–62.7)
|
57 (49–71)
|
55 (45.5–64)
|
0.004
|
Years post transplantation
| |
2.1 (1.3–5.6)
|
3 (1.7–6.2)
|
1.5 (1.1–2.4)
|
0.117
|
Vitamin D weekly dose
| | | | | |
Calcitriol (μg)
| | |
1.75 (1.75–3.5)
| | |
Cholecalciferol (IU)
| | | |
7000 (5950–10,000)
| |
No. of rejections
| |
5
|
1
|
2
|
0.275
|
CMV IgG positive recipients %
| |
52.5
|
58.1
|
56.3
|
0.894
|
ATG induction (% of patients)
| |
13
|
17
|
23
|
0.568
|
Serum Creatinine mg/dl
| |
1.5 (1.2–1.9)
|
1.6 (1.3–2.2)
|
1.2 (1.1–1.7)
|
0.155
|
Maintenance immunosuppresion (% of patients)
| | | | | |
Tac/CsA+ MPA/MMF + Steroids
| |
84
|
72
|
76
|
0.272
|
Tac/CsA+ AZA + Steroids
| |
13
|
9
|
12
| |
Tac/CsA+ everolimus/sirolimus + Steroids
| |
3
|
19
|
12
| |
C0 MPA (mg/l)
| |
1.9 (1.2–4)
|
2.6 (1.7–4)
|
2.5 (1.7–5.2)
|
0.293
|
C0 Tac (ng/ml)
| |
6 (5.2–7.8)
|
6.6 (5.7–7.3)
|
6.3 (4.8–7.3)
|
0.839
|
C0 CycA (ng/ml)
| |
116 (93–128)
|
140 (117–169)
|
63a
| |
Time of blood sampling (% of patients)
| | | | | |
Summer
|
25
|
45
|
52.6
|
31.25
| |
Autumn
|
25
|
29
|
21.1
|
56.25
|
0.252
|
Winter
|
25
|
13
|
15.8
|
6.25
| |
Spring
|
25
|
13
|
10.5
|
6.25
| |
NK cells/μl
|
176 (149–239)
|
141 (75–191)
|
106 (43–230)
|
178 (90–296)
|
0.084
|
CD8+ T cells/μl
|
377 (291–556)
|
310 (200–544)
|
417 (282–568)
|
531 (237–568)
|
0.439
|
CD4+ T cells/μl
|
762 (590–990)
|
677 (373–1043)
|
524 (357–877)
|
595 (352–1070)
|
0.144
|
Tregs/μl*
|
5.5 (4.7–8.4)
|
3.1 (1.6–5.3)
|
2.2 (1.3–4.3)
|
4.7 (3–8.5)
|
<0.001
|
Helios+ Tregs/μl*
|
2.5 (1.3–4)
|
0.7 (0.3–1.4)
|
0.6 (0.3–1.1)
|
2.2 (1.1–3.8)
|
< 0.001
|
Helios− Tregs/μl*
|
3.3 (2.5–4.2)
|
2.2 (0.9–3.9)
|
1.4 (0.9–3.1)
|
2.7 (1.1–4.6)
|
0.008
|
IFNg+ Tregs/μl*
|
0.3 (0.1–0.7)
|
0.3 (.04–0.3)
|
0.16 (0.04–0.4)
|
0.2 (0.1–0.7)
|
0.026
|
CTLA-4+ Tregs/μl*
|
1.5 (1–2.8)
|
0.4 (0.2–0.9)
|
0.35 (0–0.9)
|
1.2 (0.2–3.5)
|
< 0.001
|
Treg/CD4+ T cells %
|
0.72 (0.63–0.91)
|
0.46 (0.3–0.79)
|
0.42 (0.26–0.89)
|
0.79 (0.53–1.4)
|
< 0.001
|
Helios+ /Helios− Tregs
|
0.72 (0.41–1.1)
|
0.38 (0.27–0.61)
|
0.34 (0.21–0.65)
|
0.65 (0.39–1.4)
|
0.011
|
Treg/CD8+ T cells %
|
1.4 (0.94–2.4)
|
0.8 (0.4–1.5)
|
0.7 (0.3–1.1)
|
1.4 (0.9–1.8)
|
0.002
|