Low-dose oral cholecalciferol is associated with higher numbers of Helios+ and total Tregs than oral calcitriol in renal allograft recipients: an observational study

Table 3 CD4⁺ and Treg subset counts and ratios of Helios⁺ and total Treg cell counts in the higher-dose calcitriol group vs. cholecalciferol and non-vitamin D groups (n = 101)

	Non-vitamin D	Higher-dose Calcitriol	Cholecalciferol	P value
No. of subjects	69	16	16
CD4⁺ T cells/μl	677 (373–1043)	458 (315–879)	595 (352–1070)	0.544
Tregs/μl*	3.1 (1.6–5.3)	2.3 (1.2–4.1)	4.7 (3–8.5)	0.021
Helios⁺ Tregs/μl*	0.7 (0.3–1.4)	0.4 (0.2–1.6)	2.2 (1.1–3.8)	0.001
Helios⁻ Tregs/μl*	2.2 (0.9–3.9)	1.4 (0.9–3)	2.7 (1.1–4.6)	0.393
IFNg⁺ Tregs/μl*	0.3 (.04–0.3)	0.16 (0.06–0.4)	0.2 (0.1–0.7)	0.06
CTLA-4⁺ Tregs/μl*	0.4 (0.2–0.9)	0.25 (0–0.7)	1.2 (0.2–3.5)	0.018
Treg/CD4⁺ T cells %	0.45 (0.29–0.78)	0.44 (0.25–0.95)	0.84 (0.53–1.4)	0.006
Treg/ CD8⁺ T cells %	0.84 (0.37–1.5)	0.57 (0.27–1.2)	1.4 (0.9–1.8)	0.058
Helios⁺/ Helios⁻ Tregs	0.38 (0.27–0.61)	0.28 (0.19–0.97)	0.65 (0.38–1.4)	0.048

Tregs denoted with asterisks have the marker combination CD4⁺ CD25⁺ CD127⁻ Foxp3⁺

ISSN: 2050-6511