Skip to main content
Fig. 1 | BMC Pharmacology and Toxicology

Fig. 1

From: Functional and molecular evidence for heteromeric association of P2Y1 receptor with P2Y2 and P2Y4 receptors in mouse granulocytes

Fig. 1

Characterization of P2 responses in mice granulocytes. Granulocytes were loaded with the Ca2+ indicator fura-2. The P2X receptors agonists a αβMeATP, b BzATP and c βγMeATP did not induce increases in [Ca2+]cyt. df Average increase in [Ca2+]cyt response to these agonists. However g ATP, h ADP and i UTP were able to induce a transient increase in [Ca2+]cyt; one application of a high agonist concentration promoted desensitization of the ATP response. jl Granulocytes were pretreated for 3 h with PTX (0.05 μg/ml) or U73122 (5 μM). ATP and its analogs showed the same sensitivity to Gi-coupled protein inhibitor PTX and PLC inhibitor U73122. These results are the means ± SEM of [Ca2+]cyt increase above the resting level. *P < 0.05, ANOVA test

Back to article page
\