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Fig. 3 | BMC Pharmacology and Toxicology

Fig. 3

From: Functional and molecular evidence for heteromeric association of P2Y1 receptor with P2Y2 and P2Y4 receptors in mouse granulocytes

Fig. 3

Cross-desensitization among ATP, ADP and UTP in mice granulocytes. Granulocytes were loaded with the Ca2+ indicator fura-2. ad Addition of ATP fully inhibited responses to ADP and UTP and vice-versa. e, f ADP and UTP showed full heterologous desensitization. g, h Thapsigargin, a Ca2+-ATPase inhibitor, which releases Ca2+ from intracellular stores, showed that the lack of a second response was not due to emptying of intracellular Ca2+ stores. il fMLP, another G-protein-coupled agonist, showed the specificity of cross-desensitization between ADP and UTP. m, n ATPγS did not promote heterologous desensitization to ADP and UTP response. o, p Lower concentration of ADP and UTP reduced responses to ADP and UTP. q, r MRS2179, a specific antagonist of the P2Y1 receptor, inhibited responses to ADP but not to UTP. Granulocytes were pretreated for 30 min with MRS2179. All data are representative responses of at least four independent experiments

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