Table 1 Main taxanes found in yew needles and patient blood and their mass spectrometric characteristics
No | Compound name | [M + H]+ | MRM transitions | CE | Yew 1 | Yew 2 | Serum |
|---|---|---|---|---|---|---|---|
1 | Paclitaxel (TaxolA) | 854.2 | 854.2 > 286.1 > 105.0 | 48 | ND | ND | ND |
2 | 10-Deacetyltaxol (DAT) | 812.2 | 812.2 > 286.1 > 105.0 | 48 | ND | ND | ND |
3 | Baccatin III (BAC III) | 587.0 | 587.0 > 327 > 105.0 | 65 | + | ND | ND |
4 | 10-Deacetylbaccatin III (DAB) | 545.0 | 545.0 > 327 > 105.0 | 58 | ND | ND | ND |
5 | Cephalomannine (Taxol B) | 832.3 | 832.3 > 509 > 264 | 20 | + | ND | ND |
6 | 3,5-Dimethoxyphenol (3,5-DMP) | 155.2 | 155.2 > 123.1 > 112.1 | 25 | +++ | ++ | +++ |
7 | Monoacetyltaxine (MAT) | 568.4 | 568.4 > 194.1 > 107.2 | 40 | ++ | + | ND |
8 | Taxine B/Isotaxine B | 584.2 | 584.2 > 194.1 > 107.2 | 40 | +++ | ++ | + |
9 | Monohydroxydiacetyltaxine (MHDAT) | 626.4 | 626.4 > 194.2 > 106.8 | 45 | + | + | ND |
10 | Triacetyltaxine (TAT) | 652.3 | 652.3 > 194.1 > 107.2 | 45 | ++ | + | ND |
11 | Monohydroxytriacetyltaxine (MHTAT) | 668.4 | 668.4 > 107.2 > 103.1 | 48 | ++ | + | ND |
12 | Taxine A | 642.2 | 642.2 > 194.1 > 106.8 | 44 | + | + | ND |
13 | 2-Deacetyltaxin A | 602.2 | 602.2 > 194.1 > 106.8 | 35 | + | + | ND |
14 | Taxicatine | 317.3 | 317.3 > 155.2 | 16 | ++ | + | ND |