Fig. 4

Acute effect of various cannabinoids upon the apical uptake of 3H-FA by BeWo cells (a, b). The cell monolayers were incubated at 37 °C for 6 min with 3H-FA (50 nmol/l) at pH 7.5 (a) or pH 6.5 (b) in the absence or presence of the compounds. The tested compounds were THC (n = 9) and anandamide (Anan; n = 9). 3H-FA uptake in control cells amounted to 0.73 ± 0.08 pmol/mg prot. (n = 18) (a) and 0.81 ± 0.07 pmol/mg prot. (n = 18) (b). The results are shown as arithmetic means ± SEM. *p < 0.05 compared to control condition. Chronic (48 h) effect of various cannabinoids upon the apical uptake of 3H-FA by BeWo cells (c, d). The cell monolayers were incubated at 37 °C for 6 min with 3H-FA (50 nmol/l) at pH 7.5 (A) or pH 6.5 (b) in the absence or presence of the compounds. The tested compounds were THC (n = 9) and anandamide (Anan; n = 9). 3H-FA uptake in control cells amounted to 0.44 ± 0.03 pmol/mg prot. (n = 15) (a) and 0.62 ± 0.09 pmol/mg prot. (n = 12) (b). The results are shown as arithmetic means ± SEM. *p < 0.05 compared to control condition. [Adapted from 35]. With Permission from Karger Inc. via RightsLink