Fig. 5

Cannabidiol inhibits in vitro endothelial morphogenesis and angiogenesis. a HUVECs were incubated on a Matrigel substrate in the presence of M199 alone (Control –) or of M199 supplemented with 2 % FBS (Control +), in the absence or presence of different concentrations of CBD for 6 h at 37 °C. CBD interfered with HUVEC organization in capillary-like networks. b HUVEC spheroids, were embedded in collagen gel supplemented with VEGF (30 ng · mL-1) in the absence (Control) or in the presence of CBD (1 mM). Representative photos of each experimental group are shown. c Quantification of the sprouting. The results are expressed as the mean ± SEM of the cumulative sprout length of the capillary-like structures emerging from 24 to 26 individual spheroids per experimental group. **p < 0.01 compared to spheroids from control HUVECs, Dunnett’s t-test [Adapted from 41]. With permission from Wiley and Sons Inc. via RightsLink