Fig. 7

Anandamide inhibits neuronal differentiation via CB₁. E17 cortical neuron progenitor differentiation was performed in the presence of the indicated agents. a Immunofluorescence with anti-β-tubulin III antibody after 24 h of 5 μM AEA incubation in the absence or presence of 2 μM SR141716. b Percentage of neurons bearing neurites longer than twice (left panel) or five times (right panel) the cell body after quantification of immunofluorescence photographies. c Western blot of β-tubulin III and vimentin expression during the indicated days of cortical neuron development in the absence or presence of AEA. Loading controls were carried out with an anti-α-tubulin antibody. d Western blot of β-tubulin III (left panel) and Neu N (right panel) in cortical neuron extracts from cultures in the absence or presence of AEA and prevention of the AEA effect by SR141716. Results correspond to four different experiments. Significantly different from controls *p < 0.01 [50]. With Permission from the Journal of Biochemistry