Table 1 HPLC condition for quantitative analysis plumbagin using gradient mobile phase consisting of (A) water containing 0.1 % formic acid and (B) 100 % methanol
From: Pharmacokinetics, toxicity, and cytochrome P450 modulatory activity of plumbagin
| Time (min) | 0.1 % formic acid in water | 100 % Methanol |
|---|---|---|
| 0–2 | 60 | 40 |
| 2–2.5 | 20 | 80 |
| 2.5–7.5 | 20 | 80 |
| 7.5–8 | 60 | 40 |
| 8–10 | 60 | 40 |