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Table 1 HPLC condition for quantitative analysis plumbagin using gradient mobile phase consisting of (A) water containing 0.1 % formic acid and (B) 100 % methanol

From: Pharmacokinetics, toxicity, and cytochrome P450 modulatory activity of plumbagin

Time (min) 0.1 % formic acid in water 100 % Methanol
0–2 60 40
2–2.5 20 80
2.5–7.5 20 80
7.5–8 60 40
8–10 60 40