Table 1 HPLC condition for quantitative analysis plumbagin using gradient mobile phase consisting of (A) water containing 0.1Â % formic acid and (B) 100Â % methanol
From: Pharmacokinetics, toxicity, and cytochrome P450 modulatory activity of plumbagin
Time (min) | 0.1Â % formic acid in water | 100Â % Methanol |
|---|---|---|
0–2 | 60 | 40 |
2–2.5 | 20 | 80 |
2.5–7.5 | 20 | 80 |
7.5–8 | 60 | 40 |
8–10 | 60 | 40 |