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Table 1 HPLC condition for quantitative analysis plumbagin using gradient mobile phase consisting of (A) water containing 0.1 % formic acid and (B) 100 % methanol
From:
Pharmacokinetics, toxicity, and cytochrome P450 modulatory activity of plumbagin
Time (min)
0.1 % formic acid in water
100 % Methanol
0–2
60
40
2–2.5
20
80
2.5–7.5
20
80
7.5–8
60
40
8–10
60
40
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