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Table 2 Primer sequences used for determination of CYP1A2 and CYP3A11 mRNA expression

From: Pharmacokinetics, toxicity, and cytochrome P450 modulatory activity of plumbagin

Gene Gene accession number Amplicon size (bp) Primer sequence Melt Temperature (°C)
CYP1A2 NM_009993 149 Forward: 5′-TGGTGGAATCGGTGGCTAAC-3′ 82.50
Reverse: 5′- GACCGGGAAGAAGTCCACTG-3′
CYP3A11 NM_007818 121 Forward: 5′- ACCTGGGTGCTCCTAGCAAT-3′ 80.50
Reverse: 5′- GCACAGTGCCTAAAAATGGCA-3′
GAPDH NM_001289726 136 Forward: 5′- GGAGAGTGTTTCCTCGTCCC-3′ 84.50
Reverse: 5′- ATGAAGGGGTCGTTGATGGC-3′