Fig. 4

Effects of long-term acetaminophen exposure on neural differentiation and neurite extension. Cells were cultured on 96 well plates and continuously exposed to a range of doses of Bis1 through DIV 14. a: Quantification of total cell count (total live cells using Hoechst 33342 positive nuclei). b: Quantification of neuron density (HuC/D+). Neuron density was measured as an indicator of cell health. c, d, e: Neurites per neuron, neurite length per neuron, branch points per neuron were also measured. All data are presented as % change from untreated control wells. Total live cell count, neuron density and neurite outgrowth data are from 2 separate experiments using independent cultures (n = 12 wells total). *Concentration is significant different from control group (P < 0.05, one-way ANOVA)