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Fig. 3 | BMC Pharmacology and Toxicology

Fig. 3

From: 5-aminolevulinic acid combined with ferrous ion reduces adiposity and improves glucose tolerance in diet-induced obese mice via enhancing mitochondrial function

Fig. 3

ALA/SFC reduces lipid content in 3T3-L1 adipocytes. 3T3-L1 adipocytes were cultured in adipocyte maintenance medium with or without ALA/SFC for the indicated times, stained with Oil Red O, and the extracted Oil red O was quantified by its absorbance at 540 nm. Results are shown as means ± SD relative to the control (set to 1.0). a Cell staining was observed after ALA (1 mM)/SFC (0.5 mM) treatment for 24 h. Representative figures are shown (original magnification, × 400). b Time-dependent effects of ALA/SFC. The cells were treated with ALA (1 mM)/SFC (0.5 mM) for the indicated times and then stained with Oil Red O. **p < 0.01, ***p < 0.001 vs. control (Dunnett’s test, n = 3). c Effects of 24-h treatment of various ALA/SFC concentrations on lipid content. **p < 0.01, ***p < 0.001 vs. control (Dunnett’s test, n = 4). d Effects of 24-h treatments with ALA (1 mM) alone, SFC (0.5 mM) alone, or ALA (1 mM)/SFC (0.5 mM) in combination on lipid content. *p < 0.05, ***p < 0.001 vs. control (Bonferroni’s test, n = 4)

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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