Fig. 1

Recombined reporter gene vector pNF-κB-SEAP-Neo. a Schematic diagram of the recombined reporter gene vector pNF-κB-SEAP-Neo. The ORF of eukaryotic/ prokaryotic resistance gene NeoR/KanR was amplified from pEGFP-N1 vector and inserted into pNF-κB-SEAP vector between 2 restriction sites of SalI and AfeI to construct the recombined vector pNF-κB-SEAP-Neo. b Identification of recombined reporter gene vector pNF-κB-SEAP-Neo. Double-restriction-enzyme digestion analysis of SalI and AfeI was performed with 1% agarose gel electrophoresis. Lane 1 is the 1-kb ladder marker. Lane 2 is the restriction enzyme-digested products of the reporter gene vector pNF-κB-SEAP. Lane 3 is the restriction enzyme-digested products of amplified “Neo” transcript. Lane 4 is the restriction enzyme-digested products of the recombined reporter gene pNF-κB-SEAP-Neo