Fig. 2From: Long-term treatment of clarithromycin at a low concentration improves hydrogen peroxide-induced oxidant/antioxidant imbalance in human small airway epithelial cells by increasing Nrf2 mRNA expressionEffects of CAM pretreatment on IL-8 protein (a) and mRNA (b) levels in H2O2-stimulated SAECs. In panel a, levels of IL-8 protein were measured using the ELISA method. In panel b, IL-8 mRNA expression was measured using real-time RT-PCR. Samples were obtained from supernatants (a) or cDNA (b) of control cells, of cells stimulated with 100 μM H2O2 alone, or of cells pretreated with 1 μM, 5 μM or 10 μM CAM for 24, 48 or 72 h before stimulation with 100 μM H2O2 for 4 or 1 h, respectively. Data are presented as means ± SD of four independent experiments. *p < 0.05, **p < 0.01 vs. control cells, ## p < 0.01 vs. cells stimulated with H2O2 alone, †† p < 0.01 vs. cells pretreated with 10 μM CAMBack to article page