Fig. 3
Effects of CAM pretreatment on NF-κB (a) and p-IκBα (b) activation in SAECs stimulated with H2O2. In panel a, NF-κB activities were measured using an ELISA method. In panel b, p-IκBα protein levels were detected by western blotting (upper, representative blot images; lower, quantification of bands). Samples were obtained from nuclear proteins of control cells, of cells stimulated with 100 μM H2O2 alone, or of cells pretreated with 1 μM, 5 μM or 10 μM CAM for 24, 48 or 72 h before stimulation with 100 μM H2O2 for 0.5–1 h. The data in panel b are of cells preincubated with CAM for 72 h prior to H2O2 stimulation, and are expressed as the p-IκBα/β-actin ratio. Data are presented as means ± SD of four independent experiments. *p < 0.05, **p < 0.01 vs. control cells, ## p < 0.01 vs. cells stimulated with H2O2 alone, † p < 0.05, †† p < 0.01 vs. cells pretreated with 10 μM CAM