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Fig. 4 | BMC Pharmacology and Toxicology

Fig. 4

From: Long-term treatment of clarithromycin at a low concentration improves hydrogen peroxide-induced oxidant/antioxidant imbalance in human small airway epithelial cells by increasing Nrf2 mRNA expression

Fig. 4

Effects of CAM pretreatment on the GSH/GSSG ratio (a) and γ-GCS expression (b) in H2O2-stimulated SAECs. In panel a, GSH and GSSG were determined using the DTNB recycling method. In panel b, γ-GCS expression was detected by western blotting (upper, representative blot images; lower, quantification). Samples were obtained from supernatants (a) or cell pellets (b) of control cells, of cells stimulated with 100 μM H2O2 alone, or of cells pretreated with 1 μM, 5 μM or 10 μM CAM for 72 h before stimulation with 100 μM H2O2 for 1–2 h. The data in panel b are expressed as the γ-GCS/β-actin ratio. Data are presented as means ± SD of four independent experiments. **p < 0.01 vs. control cells, ## p < 0.01 vs. cells stimulated with H2O2 alone, †† p < 0.01 vs. cells pretreated with 10 μM CAM

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