Fig. 6
Acute application of SAL directly induced vasodilatation of middle cerebral artery isolated from normal WKY rats by inhibition of CaL channel under hyperglycemia condition. Representative diameters of middle cerebral artery were recorded before and after application of 20 mM d-glucose, 100 μM SAL, and 5 μM Bay K 8644 (the specific agonist of CaL channel), respectively (a). Summary data for average diameters before and after application of 20 mM d-glucose, 100 μM SAL, and 5 μM Bay K 8644 (b). 20 mM d-glucose induced the contraction of isolated middle cerebral artery ring and then 100 μM SAL was added as indicated dose when the contraction was stable. The SAL-induced relaxation was diminished by the addition of Bay K 8644, the specific agonist of CaL channel. Values are means ± SEM and n = 8 from 6 animals. *P < 0.05 vs. control condition and #P < 0.05 vs. high glucose condition