Fig. 2

Effect of selected E3 ligase inhibitors on growth of Plasmodium falciparum in vitro. Each point represents the mean ± SEM of three values. Minimum one thousand erythrocytes were counted for each value. a Chloroquine (CQ) was tested as standard antimalarial drug for positive control and DMSO was used as vehicle control. The ring stage synchronized CQ sensitive P. falciparum D6 strain (Pf D6) culture with high parasitemia (~6%) was treated with the antimalarial E3 ligases inhibitors namely, b JNJ 26854165 (JNJ), c HLI 373 (HLI) and (D) Nutlin-3 (Nutlin). Parasitemia was monitored at 0, 8, 16, 24, 32, 40 and 48 h post treatment as indicated. Statistically significant difference compared to corresponding hours against vehicle control (* p-value <0.05; ** p-value <0.01; ***p-value <0.001; **** p-value <0.0001)