Fig. 2

Knockdown of CSN3 decreases the protein levels of other CSN subunits a Proteins were extracted from proliferating shNT-control, shCSN3-Med or shCSN3-low stable cells lines. Total protein (20 μg) was separated by SDS-PAGE, transferred to nitrocellulose membranes, and probed for antibodies against CSN1, CSN2, CSN3, CSN5, CSN8 or GAPDH. Representative blots are shown for each antibody from samples run on a single gel. b The protein expression of each subunit was quantified and normalized to GAPDH. For each CSN subunit, the average protein intensity in shCSN3-Med and CSN3-Low cell lines was normalized to shNT-control cells set at 100. Data represent means ± SEM for at least 6 independent samples for each cell line. Data were analyzed by two-way ANOVA, ***P <0.001 compared to shNT-control