Skip to main content
Fig. 3 | BMC Pharmacology and Toxicology

Fig. 3

From: Knockdown of subunit 3 of the COP9 signalosome inhibits C2C12 myoblast differentiation via NF-KappaB signaling pathway

Fig. 3

Effect of CSN3 Knockdown on myoblast fusion. (a, b, c) Phase contrast images (20X objective) of stable cell lines grown in differentiation (DM) media from 0 to 9 days. (panels a-b) shNT-control, (panels c-d) shCSN3-Med and (panels e-f) shCSN3-Low cells. Cells were seeded in growth media (GM) until 70–80% confluent (a, panels a, c, e) and induced to differentiate for up to 9 days. (d) Immunofluorescence images. Cells were grown for 5 days in DM, fixed and stained with anti sarcomeric α actinin (red) and bisbenzimide (blue). Images were acquired with a confocal microscope (60X objective). Scale bar = 30 μm. (e) Index of fusion representative quantification. No cell fusion (NCF), *** P <0.001 significantly different from shNT-control

Back to article page