Fig. 5

Knockdown of CSN3 inhibits myoblasts proliferation. shNT-control, shCSN3-Med and shCSN3-Low cells were seeded at the same density, serum-starved for 48 h, and treated with growth media containing 10% FBS for the indicated time points (1 to 6 days). Cells were trypsinized and counted using a Coulter counter or by flow cytometry. MTT assay was performed on a 96-well plate 24 or 48 h post proliferation. a Growth curve generated from cell count obtained by Coulter counting. b Best-fit curve of the doubling times from 6 experiments, standardized to 24 h for each cell type, were significantly different (p < 0.0002). c Cells were counted by flow cytometry 48 h post growth. d MTT assay 24 or 48 h post proliferation. Values represent means ± SEM of 6 (Fig. 5a-b) and 4 (Fig. 5c) independent experiments, ** *** and #, # # # significantly different from the time matched shNT-control or from shCSN3-Med as determined by one-way (c) or two-way (a) ANOVA. **p <0.01 and ***p <0.001